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UNIVERSITY OF KWAZULU-NATAL EXAMINATIONS: JUNE 2009
SUBJECT, kind & CODE DURATION 3 HOURS INTERNAL quizzer EXTERNAL EXAMINER

FOUNDATION BIOLOGY PRACTICAL BIOL099; BIOL199 TOTAL tag 75 K. Brown, B. Keke, P. Seaman, S. Shaik, J.White Ms C. Still

CANDIDATES ARE REQUESTED, IN THEIR OWN INTERESTS, TO import LEGIBLY THIS PAPER COMPRISES FIVE PAGES AND APPENDIX A. ENSURE YOU stomach ALL OF THEM. ANSWER ALL QUESTIONS

Question 1 victimization the materials provided on the side bench, prepare a wet senesce of some of your cheek cells. Stain your wet mount apply methylene blue. 1.1 1.2 1.3 1.4 1.5 View the slide you have prepared nether high power and call a demonstrator to measure out your slide. (4) Draw and label two cells. Annotate your drawing in full. Calculate the real size of it of one cell in µm. Show your working. Calculate the magnification of your drawing. Show your working. exempt why methylene blue stain was used. (7) (2) (2) (2) 17 marks

UNIVERSITY OF KWAZULU-NATAL EXAMINATIONS COURSE & CODE: BIOL099; BIOL199 – Foundation Biology

JUNE 2009

Page 2

Question 2 witness the micrographs below and answer the questions that follow.
flagellum

Q X
chloroplast

A

Y
0.5 µm

B
2500X

P

2.



1 P Using the crustal plate bar calculate the real size (from X to Y) of the unicellular organism in micrograph A. Show your working. (2) 2.2 2.3 Calculate the real size (from P to Q) of the unicellular organism in micrograph B. Show your working. (2) explicate the difference in the size of these two organisms (A and B) by referring to patent differences in their structure. 2.4 (5)

What kind of a microscope was used to produce these micrographs? switch a reason for your answer. (2)

2.5

a.

How does organism in B nurse its energy and convert it into useable form? Refer to discernible (2)

features of the organism. b.

What kind of environment do you think this organism lives in? Give a reason for your answer. (1)

14 marks

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