Introduction- There is many important factors that will affect the yield of microbes. These factors butt joint sometimes speed up or decompress down growth extremity, or even stop the process if the conditions are too extreme. These factors are temperature, pH, and osmotic pressure. I guess the temperature or the pH factor will have the nigh effect on the microbes in the experiment.
Materials:
Nutrient culture fund of staphylococcusylococcus epidermidis, genus Serratia marcescen, and Bacillus megaterium.
2 Nutrient nutrient agar Slants
5 tubes of nutrient broth
An incubator at 25°C and 37°C and 55°C
5 petri plates
5 pipettes
1 tube of nutrient broth
1 bottle of nutrient agar
pee baths at different temperatures
Petri plates of agar 0.5% NaCl
Petri plates of agar 5% NaCl
Petri plates of agar 10% NaCl
petri plates of milk salt agar 15% NaCl
method
The method can be located in the lab manual.
We were to inoculate each one of our petri plates or our broth tubes or our tube slants with each one of the 3 cultures we were given. We then followed the directions in putting the different specimen in either the torrid water baths, in the incubator or fridge banding at the specified temperatures to find the optimum growth of the cultures.
Results
-= no growth, +- = ok growth, ++ = good growth +++ = Think growth
temperature
Temp| 5°C| 28°C| 38°C| 55°C|
StaphBacillum| +--| ++-| ++++| +--|
S. temp| 27°C| 38°C| | |
Serritia Col-Pig-| -++-+++| -+++---| | |
pH
pH| 3| 7| 10|
Staph| --| +-| ++|
Serratia| --| ++| +-|
Bacillus| --| +-| +++|
| | | |
O.P.| 0.5%| 5%| 10%|
Staph| +-| ++| ++|
Serratia| ++| +++| --|
Bacillus| +++| +++| ++-|
| | | |
Temp
Lethar Temp| | 55°C|
| 15 minute| 30 min|
Staph| ++-| ++-|
Serr ColPig| ++--| +--|
Bacillus| +++| +++|
Discussion
As we can see from the results of the lab, we can see there are many variables which can help or inhibit the...If you want to substantiate a full essay, order it on our website: Ordercustompaper.com
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